Review





Similar Products

anti pk  (Bio-Rad)
96
Bio-Rad anti pk
Anti Pk, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pk/product/Bio-Rad
Average 96 stars, based on 1 article reviews
anti pk - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
v5 antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc v5 antibody
V5 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/v5 antibody/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
v5 antibody - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
v5 tag  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc v5 tag
PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing <t>V5-tagged</t> WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization <t>to</t> <t>Vinculin</t> and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).
V5 Tag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/v5 tag/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
v5 tag - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
2 rr  (Proteintech)
95
Proteintech 2 rr
PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing <t>V5-tagged</t> WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization <t>to</t> <t>Vinculin</t> and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).
2 Rr, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2 rr/product/Proteintech
Average 95 stars, based on 1 article reviews
2 rr - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
anti v5  (Bio-Rad)
96
Bio-Rad anti v5
PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing <t>V5-tagged</t> WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization <t>to</t> <t>Vinculin</t> and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).
Anti V5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti v5/product/Bio-Rad
Average 96 stars, based on 1 article reviews
anti v5 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
mouse monoclonal anti v5 tag antibody  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc mouse monoclonal anti v5 tag antibody
PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing <t>V5-tagged</t> WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization <t>to</t> <t>Vinculin</t> and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).
Mouse Monoclonal Anti V5 Tag Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti v5 tag antibody/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
mouse monoclonal anti v5 tag antibody - by Bioz Stars, 2026-05
97/100 stars
  Buy from Supplier
rabbit anti v5  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti v5
PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing <t>V5-tagged</t> WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization <t>to</t> <t>Vinculin</t> and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).
Rabbit Anti V5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti v5/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
rabbit anti v5 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

Image Search Results


PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing V5-tagged WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization to Vinculin and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).

Journal: The Journal of Biological Chemistry

Article Title: The protein phosphatase 2A-B56α complex regulates N-Myc degradation in neuroblastoma

doi: 10.1016/j.jbc.2026.111298

Figure Lengend Snippet: PP2A activity is dependent on the N-Myc phosphorylation sites S62 and T58. A , Kelly cells were transduced with pLenti6.3 expressing V5-tagged WT N-Myc and mutant S62D or T58A N-Myc. Cells were treated with 25 μg/ml cycloheximide in a chase experiment to assess protein stability over 60 min by immunoblot of whole cell lysate for V5-tagged N-Myc. B , quantification of V5-tagged N-Myc plotted after normalization to Vinculin and the untreated control. Data plotted as mean ± S.D. are from four independent experiments ( n = 4), and half-lives were determined from exponential decay curve fitting. C , Kelly cells expressing the WT, S62D, and T58A N-Myc are treated with DMSO or 20 μM DT-061 for 3 h and immunoblotted for V5-tagged N-Myc, representative images are presented. D , quantification of V5-tagged N-Myc after normalization to Vinculin and DMSO controls from three independent experiments ( n = 3). Statistical significance was determined by two-way ANOVA; ∗ p < 0.05; ns, not significant. E , representative images of clonogenic assays of Kelly WT and S62D N-Myc plated at low density and treated with DT-061 for 14 days. F , colony formation assays from panel E were quantified by absorbance of dissolved crystal violet stained colonies, and plotted data represent the mean ± S.D. of three independent biological replicates (two technical replicates for each biological replicate).

Article Snippet: Membranes were blocked in 5% non-fat milk/TBST for 1 hour and incubated overnight at 4 °C with primary antibodies: c-Myc (ABclonal, A19032, 1:1000), phospho S62 c-Myc (Abcam, ab185656, 1:1000), N-Myc (Abcam, ab16898, 1:1000), Vinculin (Santa Cruz Biotech, sc-73614, 1:5,000), GAPDH (Santa Cruz Biotech, sc-32233, 1:2000), V5-tag (Cell Signaling Technology, 13,202, 1:1000), AP2β (Cell Signaling Technology, 2509, 1:1000), GATA3 (Cell Signaling Technology, 5852, 1:1,000), PHOX2B (Santa Cruz Biotech, sc-376997, 1:1,000), PARP (Cell Signaling Technology, 9542L, 1:1000), Cleaved caspase-3 (Cell Signaling Technology, 9664L, 1:1,000), and PPP2R2A (Santa Cruz Biotech, sc-81606, 1:1000).

Techniques: Activity Assay, Phospho-proteomics, Transduction, Expressing, Mutagenesis, Western Blot, Control, Staining